Journal: Theranostics

Article Title: High OGT activity is essential for MYC-driven proliferation of prostate cancer cells

doi: 10.7150/thno.30834

Figure Lengend Snippet: Identification of transcription factors that bind to O-GlcNAc marked chromatin. A) Distribution of differently modified histone 3 reads within ±2kb of O-GlcNAc peak. B) . The top 3 motifs enriched for the O-GlcNAc ChIP-seq data showing similarity with known previously identified transcription factor motifs from published datasets. C) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-seq data reported in this study. D) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-exo dataset reported previously (GSE73994). E) OSMI-2 disrupts interaction between MYC and HCF-1. Immunoprecipitation (IP) of MYC. IgG denotes negative control. MYC was overexpressed by addition of doxycycline using the LNCaP-MYC cell line for 4 hours either in the presence or absence of 40µM OSMI-2. Experiment was repeated four times. F) Knockout of OGT disrupts the interaction between MYC and HCF-1. Experiment was performed in a mouse embryonic fibroblast cell line that has been genetically engineered to enable removal of OGT gene by addition of 0.5µM Tamoxifen (Tam). After two days of DMSO- or Tam-treatments, cell lysates were prepared and used for immunoprecipitation. Data shown is representative of two biological replicates. G) Overlap of O-GlcNAc (this study), MYC (this study) and HCF-1 (ENCSR000ECH) ChIP-seq data.

Article Snippet: Tamoxifen-inducible OGT knockout mouse embryonic fibroblast (MEF) cell line was obtained from Dr. Natasha Zachara at the CardioPEG CoreC4 (NHLBI P01 HL107153) at Johns Hopkins University School of Medicine .

Techniques: Modification, ChIP-sequencing, Immunoprecipitation, Negative Control, Knock-Out

Journal:

Article Title: A Cajal body-independent pathway for telomerase trafficking in mice

doi: 10.1016/j.yexcr.2010.07.001

Figure Lengend Snippet: Mouse embryonic fibroblast (MEF-26, 3T3), n2a neuroblastoma, and c2c12 myoblast cell lines were co-analyzed for mTR (detected by FISH, red) and coilin (marker protein for Cajal bodies, detected by IF, green). Merge panels indicate an overlay of mTR and coilin panels. Open arrowheads point to Cajal bodies that do not overlap with mTR foci. Scale bar, 10 microns.

Article Snippet: We would like to thank Tammy Morrish and Carol Greider (Johns Hopkins University, Baltimore, MD) and Greg Matera (University of North Carolina, Chapel Hill, NC) for providing the mTR knockout and coilin knockout MEF cell lines, respectively.

Techniques: Marker

Journal:

Article Title: A Cajal body-independent pathway for telomerase trafficking in mice

doi: 10.1016/j.yexcr.2010.07.001

Figure Lengend Snippet: A. mTR foci do not correspond to known markers for Cajal bodies. mTR FISH (red, mTR panels) was performed in tandem with one of three markers for Cajal bodies: U85 scaRNA (top row, detected by FISH), SMN (middle row, detected by IF, signal present in Cajal bodies and cytoplasm), or Nopp140 (bottom row, detected by IF, signal present in Cajal bodies and nucleoli). Arrowheads denote Cajal bodies; open arrowheads point to mTR foci that do not localize to Cajal bodies. B. mTR localizes to intranuclear foci in MEF cells derived from coilin knockout (KO) mice. mTR FISH was performed on coilin KO MEF cells. Open arrowheads point to mTR foci. DAPI was used to stain the DNA. Scale bars, 10 microns.

Article Snippet: We would like to thank Tammy Morrish and Carol Greider (Johns Hopkins University, Baltimore, MD) and Greg Matera (University of North Carolina, Chapel Hill, NC) for providing the mTR knockout and coilin knockout MEF cell lines, respectively.

Techniques: Derivative Assay, Knock-Out, Staining

Journal:

Article Title: A Cajal body-independent pathway for telomerase trafficking in mice

doi: 10.1016/j.yexcr.2010.07.001

Figure Lengend Snippet: mTR FISH (red) and TPP1 IF (green, marker for telomeres) were performed on MEF cells derived from wild type (WT) and coilin −/− (coilin KO) MEFs. Merge panels display an overlay of mTR and TPP1 signals. Arrowheads denote foci where both mTR and TPP1 signals overlap. Scale bar, 10 microns.

Article Snippet: We would like to thank Tammy Morrish and Carol Greider (Johns Hopkins University, Baltimore, MD) and Greg Matera (University of North Carolina, Chapel Hill, NC) for providing the mTR knockout and coilin knockout MEF cell lines, respectively.

Techniques: Marker, Derivative Assay

Journal:

Article Title: A Cajal body-independent pathway for telomerase trafficking in mice

doi: 10.1016/j.yexcr.2010.07.001

Figure Lengend Snippet: A. hTR co-localizes with Cajal bodies in mouse cells. Human telomerase RNA (hTR) was expressed in n2a and MEF-26 mouse cell lines and co-analyzed for hTR (detected by FISH, red panels) and coilin (detected by IF, green panels). Merge panels display an overlay of hTR and coilin signals. B. hTR co-localizes with telomeres in mouse cells. hTR-transfected n2a and MEF-26 mouse cells were examined for hTR (detected by FISH, red panels) and telomere (detected by FISH, green panels) signals. Merge panels display an overlay of hTR and telomere signals.

Article Snippet: We would like to thank Tammy Morrish and Carol Greider (Johns Hopkins University, Baltimore, MD) and Greg Matera (University of North Carolina, Chapel Hill, NC) for providing the mTR knockout and coilin knockout MEF cell lines, respectively.

Techniques: Transfection

Journal: eLife

Article Title: PHAROH lncRNA regulates Myc translation in hepatocellular carcinoma via sequestering TIAR

doi: 10.7554/eLife.68263

Figure Lengend Snippet:

Article Snippet: Cell line ( Mus musculus ) , MEF , MTI-Global Stem , Cat# GSC-6601G , Irradiated feeder MEFs.

Techniques: Recombinant, Irradiation, Control, Plasmid Preparation, Expressing, Transfection, Construct, Negative Control, Luciferase, Sequencing, Amplification, Reverse Transcription, ISH Cell Assay, Bicinchoninic Acid Protein Assay, Proliferation Assay, Reporter Assay, Software, Cloning